Journal: Regenerative Therapy

Article Title: Influence of donor–recipient age differences in allogenic rat thyroid transplantation

doi: 10.1016/j.reth.2025.101047

Figure Lengend Snippet: Functional, histological and gene expression analysis of thyroids in 3-week-old and retired rats. (A) FreeT4 levels in serum of 3-week-old (n = 5) and retired rats (n = 5). N.S., not significant. (B) mRNA expression levels of thyroid differentiation marker genes in thyroids from 3-week-old rats (n = 3) and retired rats (n = 3). Expression levels of thyroid genes were normalized to Gapdh . ∗: P < 0.05. N.S., not significant. (C) Thyroid of a retired rat (left) and a 3-week-old rat (right). HE staining (top), TG (2nd, red), Hoechst33342 (2nd, blue), vimentin (3rd, green), Hoechst33342 (3rd, blue), collagen IV (bottom, red), and TTF-1 (bottom, green). Scale bar, 100 μm in HE-stained images, 50 μm in immunofluorescent images.

Article Snippet: Mouse anti-rat thyroid transcription factor-1 (TTF-1) (M3575; Dako, 1:200–1:100), rabbit anti-rat thyroglobulin (TG) (ab156008; Abcam Limited, Cambridge, UK, 1:250–1:100), rabbit anti-rat collagen IV (ab6586; Abcam Limited, 1:200), and mouse anti-rat vimentin conjugated to FITC (sc6260; Santa Cruz Biotechnology, Dallas, TX, USA, 1:100) primary antibodies were incubated at 4 °C overnight.

Techniques: Functional Assay, Gene Expression, Expressing, Marker, Staining

Journal: Regenerative Therapy

Article Title: Influence of donor–recipient age differences in allogenic rat thyroid transplantation

doi: 10.1016/j.reth.2025.101047

Figure Lengend Snippet: Histological evaluation of transplanted thyroids 1 month after age-matched or age-mismatched transplantation , and of thyroid follicular structures at 6 months post-transplantation of retired rat thyroids into 3-week-old rats. (A) Schematic of the age-matched and age-mismatched thyroid transplantation protocol. (B) Thyroid grafts 1 month after transplantation (n = 3 per group, independent samples). The transplanted thyroids of (a) retired rats into retired rats, (b) 3-week-old rats into 3-week-old rats, (c) retired rats into 3-week-old rats, and (d) 3-week-old rats into retired rats. Upper, HE staining; lower; Hoechst33342 (blue) and Tg (red). Scale bar = 100 μm in the HE-stained images, 30 μm in immunofluorescent images. (C) (a) Thyroid grafts of retired rats transplanted into 3-week-old rats and (b) those of 3-week-old rats transplanted into retired rats 1 month after transplantation (n = 3 per group, independent samples). Upper, vimentin (green) and Hoechst33342 (blue); lower, collagen IV (red) and TTF-1 (green). Scale bar = 50 μm. (D) Thyroid grafts from retired rats into 3-week-old rats 6 months after transplantation (n = 3, independent animals). HE staining (top), TG (2nd, red) and Hoechst33342 (2nd, blue), vimentin (3rd, green) and Hoechst33342 (3rd, blue), collagen IV (bottom, red) and TTF-1 (bottom, green). Scale bar = 100 μm in the HE-stained images, 50 μm in immunofluorescent images.

Article Snippet: Mouse anti-rat thyroid transcription factor-1 (TTF-1) (M3575; Dako, 1:200–1:100), rabbit anti-rat thyroglobulin (TG) (ab156008; Abcam Limited, Cambridge, UK, 1:250–1:100), rabbit anti-rat collagen IV (ab6586; Abcam Limited, 1:200), and mouse anti-rat vimentin conjugated to FITC (sc6260; Santa Cruz Biotechnology, Dallas, TX, USA, 1:100) primary antibodies were incubated at 4 °C overnight.

Techniques: Transplantation Assay, Staining